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7 amino actinomycin d 7 aad staining solution  (Thermo Fisher)


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    Structured Review

    Thermo Fisher 7 amino actinomycin d 7 aad staining solution
    7 Amino Actinomycin D 7 Aad Staining Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/7+amino+actinomycin+d/Actinomycin+D/pmc13049938-326-10-15
    Average 97 stars, based on 1 article reviews
    7 amino actinomycin d 7 aad staining solution - by Bioz Stars, 2026-10
    97/100 stars

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    Saline:

    Article Title: Nonclinical evaluation of renizgamglogene autogedtemcel for SCD and TDT
    Article Snippet: CD34 + cells at day 2 post-electroporation were stained with anti-human CD34-BV421, anti-human CD38-BV605, anti-human CD123-BV711, anti-human CD45RA-fluorescein isothiocyanate (FITC), and anti-human CD90-R-phycoerythrin-cyanine7 (PE-Cy7) (all from BioLegend, San Diego, CA) for 30 min at 4°C. .. Samples were washed with phosphate-buffered saline (PBS)-supplemented 0.5% bovine serum albumin (BSA) (Miltenyi Biotec, Auburn, CA) and resuspended in PBS with 7-amino-actinomycin D (eBioscience, San Diego, CA) for dead cell exclusion. .. HSCs (CD34 + CD38 low CD90 + CD45RA − ), MPPs (CD34 + CD38 low CD90 − CD45RA − ), and CMPs (CD34 + CD38 high CD123 + CD45RA − ) were sorted on a BD FACSAria Fusion cell sorter (BD Biosciences, Franklin Lakes, NJ).

    Isolation:

    Article Title: Efficacy of intranasal adenovirus vector vaccine is attenuated by type I IFN‒induced NK cell activation
    Article Snippet: .. Isolated cells were treated with mouse FcR blocking reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) at 4◦C for 5 min. Fluorescently labeled antibodies shown in Table 2 were then added and stained at 4◦C for 30 min. Dead cells were excluded by staining with 7-amino-actinomycin D (Thermo Fisher Scientific). .. The stained samples were acquired using a MACSQuant flow cytometer (Miltenyi Biotec), and samples were analyzed with FlowJo software v.10 (FlowJo, LLC, Ashland, OR).

    Blocking Assay:

    Article Title: Efficacy of intranasal adenovirus vector vaccine is attenuated by type I IFN‒induced NK cell activation
    Article Snippet: .. Isolated cells were treated with mouse FcR blocking reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) at 4◦C for 5 min. Fluorescently labeled antibodies shown in Table 2 were then added and stained at 4◦C for 30 min. Dead cells were excluded by staining with 7-amino-actinomycin D (Thermo Fisher Scientific). .. The stained samples were acquired using a MACSQuant flow cytometer (Miltenyi Biotec), and samples were analyzed with FlowJo software v.10 (FlowJo, LLC, Ashland, OR).

    Labeling:

    Article Title: Efficacy of intranasal adenovirus vector vaccine is attenuated by type I IFN‒induced NK cell activation
    Article Snippet: .. Isolated cells were treated with mouse FcR blocking reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) at 4◦C for 5 min. Fluorescently labeled antibodies shown in Table 2 were then added and stained at 4◦C for 30 min. Dead cells were excluded by staining with 7-amino-actinomycin D (Thermo Fisher Scientific). .. The stained samples were acquired using a MACSQuant flow cytometer (Miltenyi Biotec), and samples were analyzed with FlowJo software v.10 (FlowJo, LLC, Ashland, OR).

    Staining:

    Article Title: Efficacy of intranasal adenovirus vector vaccine is attenuated by type I IFN‒induced NK cell activation
    Article Snippet: .. Isolated cells were treated with mouse FcR blocking reagent (Miltenyi Biotec, Bergisch Gladbach, Germany) at 4◦C for 5 min. Fluorescently labeled antibodies shown in Table 2 were then added and stained at 4◦C for 30 min. Dead cells were excluded by staining with 7-amino-actinomycin D (Thermo Fisher Scientific). .. The stained samples were acquired using a MACSQuant flow cytometer (Miltenyi Biotec), and samples were analyzed with FlowJo software v.10 (FlowJo, LLC, Ashland, OR).

    Article Title: Methods and compositions for inhibiting CD32B expressing cells in IgG4-related diseases
    Article Snippet: .. After incubation, cells were harvested and stained with PE-conjugated annexin-V (Biovision, Mountain View, CA) and 7-amino-actinomycin D (7-AAD, Invitrogen, Carlsbad, CA) at 5 μg/ml. .. The annexin-V-positive/7-AAD-negative cells were acquired using a FACSCanto II flow cytometer, and analyzed with FACSDiva 5 analysis software (BD Biosciences).

    Article Title: Myeloid HDAC7 drives liver inflammation and systemic glucose dysregulation during diet‐induced obesity
    Article Snippet: For immunophenotyping, spleen samples were stained in 50% CD16/CD32 hybridoma 2.4G2 supernatant for blocking and a fluorescent antibody cocktail containing anti‐mouse CD45‐Pacific Blue (BioLegend, Cat#103126, clone 30‐F11, 1/200 dilution), anti‐mouse CD3ε‐FITC (BioLegend, cat# 100306, clone 145‐2C11, 1/200 dilution), anti‐mouse/human B220‐APC‐Cy7 (BioLegend, cat# 03224, clone RA3‐6B2, 1/200 dilution) and anti‐mouse/human CD11b‐PerCP‐Cy5.5 (BioLegend, cat# 101228, clone M1/70, 1/200 dilution) was used to measure numbers of T, B and total myeloid cells. .. 7‐amino‐actinomycin D (7AAD; ThermoFisher Scientific, cat# A1310, 1/10 dilution) or fixable viability stain (FVS)700 (BD Bioscience, cat# 564997, 1/10 000 dilution) was added to all stained samples for dead cell exclusion. .. Samples were analysed on a Cytoflex (Beckman Coulter) flow cytometer equipped with 640, 561, 488 and 405 nm lasers.

    other:

    Article Title: Single-nucleus brain transcriptomics reveals microglia dysfunction in multiple system atrophy.
    Article Snippet: The tissue was homogenized in a precooled Dounce homogenizer containing ice-cooled homogenization buffer (250 mM sucrose, 25 mM KCl, 5 mM MgCl2, 10 mM Tris buffer, pH 8.0, 1 μM DTT, 1x protease inhibitor (Roche, Switzerland), 0.4 U/μL RNAse inhibitor (Takara, USA), 0.2 U/μL Superasin inhibitor (Invitrogen, USA), and 0.1% Triton X-100) using both loose and tight pestles before filtering using a 70 μm filter (Millipore).

    Incubation:

    Article Title: Methods and compositions for inhibiting CD32B expressing cells in IgG4-related diseases
    Article Snippet: .. After incubation, cells were harvested and stained with PE-conjugated annexin-V (Biovision, Mountain View, CA) and 7-amino-actinomycin D (7-AAD, Invitrogen, Carlsbad, CA) at 5 μg/ml. .. The annexin-V-positive/7-AAD-negative cells were acquired using a FACSCanto II flow cytometer, and analyzed with FACSDiva 5 analysis software (BD Biosciences).

    Article Title: Biases introduced by Ficoll-based isolation in acute myeloid leukemia sample analyses support the use of hemolysis
    Article Snippet: .. Cells were washed with PBS and incubated for 5 mins with 7-amino-actinomycin D (7-AAD, cat: 00-6993-50; eBioscience) before analysis on a cytoFLEX flow cytometer (Beckman Coulter (BC)). ..

    Flow Cytometry:

    Article Title: Biases introduced by Ficoll-based isolation in acute myeloid leukemia sample analyses support the use of hemolysis
    Article Snippet: .. Cells were washed with PBS and incubated for 5 mins with 7-amino-actinomycin D (7-AAD, cat: 00-6993-50; eBioscience) before analysis on a cytoFLEX flow cytometer (Beckman Coulter (BC)). ..



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